fap-α rabbit polyclonal antibody Search Results


anti fap  (OriGene)
90
OriGene anti fap
Anti Fap, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fap-%CE%B1+rabbit+polyclonal+antibody/Fibroblast+activation+protein%2C+alpha+(FAP)+Rabbit+Polyclonal+Antibody/pmc08184678-68-22-23
Average 90 stars, based on 1 article reviews
anti fap - by Bioz Stars, 2026-10
90/100 stars
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expression vectors  (OriGene)
90
OriGene expression vectors
Expression Vectors, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fap-%CE%B1+rabbit+polyclonal+antibody/Fibroblast+activation+protein%2C+alpha+(FAP)+Rabbit+Polyclonal+Antibody/pm28379093-302-18-37
Average 90 stars, based on 1 article reviews
expression vectors - by Bioz Stars, 2026-10
90/100 stars
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fapa antibody  (Cusabio)
93
Cusabio fapa antibody
( A and B ) CAFs in tumors of vehicle- and CSF1Ri-treated animals were visualized upon <t>FAPA</t> staining. ( A ) Representative pictures. Scale bar: 50 μm. ( B ) Results of image analysis. Data presented as mean ± SEM. LLC: n = 4 for both groups. MC38: n = 5 for both groups. * P < 0.05 compared with vehicle by 2-tailed Student’s t test. HPF, high-power field. ( C ) Activated CAFs isolated from LLC or MC38 tumors were treated with vehicle or 6700 nM CSF1Ri overnight and subsequently loaded with 7 × 10 4 tumor cells/well; 8 hours later 2 × 10 5 M1 macrophages/well were added to the tumor cell–CAF cocultures; 24 hours later, tumor cells were counted. ( D and E ) CD45 + CD11b + F4/80 + macrophages were analyzed for IL-10 ( D ) and IL-12 ( E ) expression. Data presented as mean ± SEM, 2 independent experiments. LLC: vehicle-treated CAFs n = 5, CSF1Ri-treated CAFs n = 5. MC38: vehicle-treated CAFs n = 7, CSF1Ri-treated CAFs n = 7. * P < 0.05 compared with vehicle-treated CAFs by 2-tailed Student’s t test. ( F and G ) Isolated CAFs from LLC and MC38 tumors (treated with vehicle or CSF1Ri overnight) were cocultured with macrophages for 48 hours. Macrophages were isolated and mRNA levels of Vegfa ( F ) and Il6 ( G ) were quantified by real-time PCR. Data presented as mean ± SEM. n = 7 for both groups, * P < 0.05 compared with indicated group by 2-tailed Student’s t test. ( H ) Isolated CAFs from LLC or MC38 tumors were treated with vehicle, CSF1Ri, CSF1, or IL-34 or pretreated with CSF1Ri for 2 hours and then CSF1 was added; 24 hours later, MIP2 levels were quantified in supernatants. Results were normalized to total protein. Data presented as mean ± SEM. Vehicle n = 6, CSF1Ri n = 6, CSF1 n = 5, CSF1Ri+CSF1 n = 4, IL-34 n = 5 (3 independent experiments). * P < 0.05 compared with indicated group by 1-way ANOVA (with Tukey’s post hoc test).
Fapa Antibody, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fap-%CE%B1+rabbit+polyclonal+antibody/Rabbit+anti-+FAP+Polyclonal+Antibody/pmc08986064-133-9-12
Average 93 stars, based on 1 article reviews
fapa antibody - by Bioz Stars, 2026-10
93/100 stars
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anti fap1α  (Cusabio)
94
Cusabio anti fap1α
( A and B ) CAFs in tumors of vehicle- and CSF1Ri-treated animals were visualized upon <t>FAPA</t> staining. ( A ) Representative pictures. Scale bar: 50 μm. ( B ) Results of image analysis. Data presented as mean ± SEM. LLC: n = 4 for both groups. MC38: n = 5 for both groups. * P < 0.05 compared with vehicle by 2-tailed Student’s t test. HPF, high-power field. ( C ) Activated CAFs isolated from LLC or MC38 tumors were treated with vehicle or 6700 nM CSF1Ri overnight and subsequently loaded with 7 × 10 4 tumor cells/well; 8 hours later 2 × 10 5 M1 macrophages/well were added to the tumor cell–CAF cocultures; 24 hours later, tumor cells were counted. ( D and E ) CD45 + CD11b + F4/80 + macrophages were analyzed for IL-10 ( D ) and IL-12 ( E ) expression. Data presented as mean ± SEM, 2 independent experiments. LLC: vehicle-treated CAFs n = 5, CSF1Ri-treated CAFs n = 5. MC38: vehicle-treated CAFs n = 7, CSF1Ri-treated CAFs n = 7. * P < 0.05 compared with vehicle-treated CAFs by 2-tailed Student’s t test. ( F and G ) Isolated CAFs from LLC and MC38 tumors (treated with vehicle or CSF1Ri overnight) were cocultured with macrophages for 48 hours. Macrophages were isolated and mRNA levels of Vegfa ( F ) and Il6 ( G ) were quantified by real-time PCR. Data presented as mean ± SEM. n = 7 for both groups, * P < 0.05 compared with indicated group by 2-tailed Student’s t test. ( H ) Isolated CAFs from LLC or MC38 tumors were treated with vehicle, CSF1Ri, CSF1, or IL-34 or pretreated with CSF1Ri for 2 hours and then CSF1 was added; 24 hours later, MIP2 levels were quantified in supernatants. Results were normalized to total protein. Data presented as mean ± SEM. Vehicle n = 6, CSF1Ri n = 6, CSF1 n = 5, CSF1Ri+CSF1 n = 4, IL-34 n = 5 (3 independent experiments). * P < 0.05 compared with indicated group by 1-way ANOVA (with Tukey’s post hoc test).
Anti Fap1α, supplied by Cusabio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fap-%CE%B1+rabbit+polyclonal+antibody/Rabbit+anti-+FAP+Polyclonal+Antibody/pm40426868-68-13-15
Average 94 stars, based on 1 article reviews
anti fap1α - by Bioz Stars, 2026-10
94/100 stars
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rabbit polyclonal anti-fap antibody  (GeneTex)
90
GeneTex rabbit polyclonal anti-fap antibody
( A and B ) CAFs in tumors of vehicle- and CSF1Ri-treated animals were visualized upon <t>FAPA</t> staining. ( A ) Representative pictures. Scale bar: 50 μm. ( B ) Results of image analysis. Data presented as mean ± SEM. LLC: n = 4 for both groups. MC38: n = 5 for both groups. * P < 0.05 compared with vehicle by 2-tailed Student’s t test. HPF, high-power field. ( C ) Activated CAFs isolated from LLC or MC38 tumors were treated with vehicle or 6700 nM CSF1Ri overnight and subsequently loaded with 7 × 10 4 tumor cells/well; 8 hours later 2 × 10 5 M1 macrophages/well were added to the tumor cell–CAF cocultures; 24 hours later, tumor cells were counted. ( D and E ) CD45 + CD11b + F4/80 + macrophages were analyzed for IL-10 ( D ) and IL-12 ( E ) expression. Data presented as mean ± SEM, 2 independent experiments. LLC: vehicle-treated CAFs n = 5, CSF1Ri-treated CAFs n = 5. MC38: vehicle-treated CAFs n = 7, CSF1Ri-treated CAFs n = 7. * P < 0.05 compared with vehicle-treated CAFs by 2-tailed Student’s t test. ( F and G ) Isolated CAFs from LLC and MC38 tumors (treated with vehicle or CSF1Ri overnight) were cocultured with macrophages for 48 hours. Macrophages were isolated and mRNA levels of Vegfa ( F ) and Il6 ( G ) were quantified by real-time PCR. Data presented as mean ± SEM. n = 7 for both groups, * P < 0.05 compared with indicated group by 2-tailed Student’s t test. ( H ) Isolated CAFs from LLC or MC38 tumors were treated with vehicle, CSF1Ri, CSF1, or IL-34 or pretreated with CSF1Ri for 2 hours and then CSF1 was added; 24 hours later, MIP2 levels were quantified in supernatants. Results were normalized to total protein. Data presented as mean ± SEM. Vehicle n = 6, CSF1Ri n = 6, CSF1 n = 5, CSF1Ri+CSF1 n = 4, IL-34 n = 5 (3 independent experiments). * P < 0.05 compared with indicated group by 1-way ANOVA (with Tukey’s post hoc test).
Rabbit Polyclonal Anti Fap Antibody, supplied by GeneTex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fap-%CE%B1+rabbit+polyclonal+antibody/rabbit+polyclonal+anti+fap+antibody/pmc09306099-141-8-12
Average 90 stars, based on 1 article reviews
rabbit polyclonal anti-fap antibody - by Bioz Stars, 2026-10
90/100 stars
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fibroblast activation protein alpha fap rabbit polyclonal antibody  (OriGene)
N/A
Fibroblast activation protein alpha FAP rabbit polyclonal antibody Aff Purified
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rabbit anti- fap polyclonal antibody  (Cusabio)
N/A
Rabbit anti-Human FAP Polyclonal Antibody
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rabbit anti-human fap polyclonal antibody  (Cusabio)
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Rabbit anti-Human FAP Polyclonal Antibody
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Image Search Results


( A and B ) CAFs in tumors of vehicle- and CSF1Ri-treated animals were visualized upon FAPA staining. ( A ) Representative pictures. Scale bar: 50 μm. ( B ) Results of image analysis. Data presented as mean ± SEM. LLC: n = 4 for both groups. MC38: n = 5 for both groups. * P < 0.05 compared with vehicle by 2-tailed Student’s t test. HPF, high-power field. ( C ) Activated CAFs isolated from LLC or MC38 tumors were treated with vehicle or 6700 nM CSF1Ri overnight and subsequently loaded with 7 × 10 4 tumor cells/well; 8 hours later 2 × 10 5 M1 macrophages/well were added to the tumor cell–CAF cocultures; 24 hours later, tumor cells were counted. ( D and E ) CD45 + CD11b + F4/80 + macrophages were analyzed for IL-10 ( D ) and IL-12 ( E ) expression. Data presented as mean ± SEM, 2 independent experiments. LLC: vehicle-treated CAFs n = 5, CSF1Ri-treated CAFs n = 5. MC38: vehicle-treated CAFs n = 7, CSF1Ri-treated CAFs n = 7. * P < 0.05 compared with vehicle-treated CAFs by 2-tailed Student’s t test. ( F and G ) Isolated CAFs from LLC and MC38 tumors (treated with vehicle or CSF1Ri overnight) were cocultured with macrophages for 48 hours. Macrophages were isolated and mRNA levels of Vegfa ( F ) and Il6 ( G ) were quantified by real-time PCR. Data presented as mean ± SEM. n = 7 for both groups, * P < 0.05 compared with indicated group by 2-tailed Student’s t test. ( H ) Isolated CAFs from LLC or MC38 tumors were treated with vehicle, CSF1Ri, CSF1, or IL-34 or pretreated with CSF1Ri for 2 hours and then CSF1 was added; 24 hours later, MIP2 levels were quantified in supernatants. Results were normalized to total protein. Data presented as mean ± SEM. Vehicle n = 6, CSF1Ri n = 6, CSF1 n = 5, CSF1Ri+CSF1 n = 4, IL-34 n = 5 (3 independent experiments). * P < 0.05 compared with indicated group by 1-way ANOVA (with Tukey’s post hoc test).

Journal: JCI Insight

Article Title: CSF1/CSF1R signaling mediates malignant pleural effusion formation

doi: 10.1172/jci.insight.155300

Figure Lengend Snippet: ( A and B ) CAFs in tumors of vehicle- and CSF1Ri-treated animals were visualized upon FAPA staining. ( A ) Representative pictures. Scale bar: 50 μm. ( B ) Results of image analysis. Data presented as mean ± SEM. LLC: n = 4 for both groups. MC38: n = 5 for both groups. * P < 0.05 compared with vehicle by 2-tailed Student’s t test. HPF, high-power field. ( C ) Activated CAFs isolated from LLC or MC38 tumors were treated with vehicle or 6700 nM CSF1Ri overnight and subsequently loaded with 7 × 10 4 tumor cells/well; 8 hours later 2 × 10 5 M1 macrophages/well were added to the tumor cell–CAF cocultures; 24 hours later, tumor cells were counted. ( D and E ) CD45 + CD11b + F4/80 + macrophages were analyzed for IL-10 ( D ) and IL-12 ( E ) expression. Data presented as mean ± SEM, 2 independent experiments. LLC: vehicle-treated CAFs n = 5, CSF1Ri-treated CAFs n = 5. MC38: vehicle-treated CAFs n = 7, CSF1Ri-treated CAFs n = 7. * P < 0.05 compared with vehicle-treated CAFs by 2-tailed Student’s t test. ( F and G ) Isolated CAFs from LLC and MC38 tumors (treated with vehicle or CSF1Ri overnight) were cocultured with macrophages for 48 hours. Macrophages were isolated and mRNA levels of Vegfa ( F ) and Il6 ( G ) were quantified by real-time PCR. Data presented as mean ± SEM. n = 7 for both groups, * P < 0.05 compared with indicated group by 2-tailed Student’s t test. ( H ) Isolated CAFs from LLC or MC38 tumors were treated with vehicle, CSF1Ri, CSF1, or IL-34 or pretreated with CSF1Ri for 2 hours and then CSF1 was added; 24 hours later, MIP2 levels were quantified in supernatants. Results were normalized to total protein. Data presented as mean ± SEM. Vehicle n = 6, CSF1Ri n = 6, CSF1 n = 5, CSF1Ri+CSF1 n = 4, IL-34 n = 5 (3 independent experiments). * P < 0.05 compared with indicated group by 1-way ANOVA (with Tukey’s post hoc test).

Article Snippet: CAFs were isolated from LLC or MC38 tumors by FAPA antibody–loaded (CSB-PA008191, Cusabio Technology) magnetic beads (MojoSort Streptavidin Nanobeads, BioLegend) as previously reported ( ).

Techniques: Staining, Isolation, Expressing, Real-time Polymerase Chain Reaction